Preliminary Study on Preventing Explant from Browning in Tissue Culture of Ficus carica

[Objective] The study aimed to discuss the best method for preventing explant from browning in Ficus carica tissue culture.[Method] With the terminal bud of F.carica young tree as tested material,the effect of 2 kinds of explants,4 disinfection methods,a same sort of disinfectant with different sanitizing time,5 hormone combinations,3 anti-browning agents on explant browning in F.carica tissue culture.[Result] Compared with the terminal buds that just grew young leaves,the terminal buds without sprouting was suitable to act as anti-browning explant.The best disinfection method was to immerse the explant in Vc solution at 1 000 mg/L for 30 min.First,and then sterilize with 0.1% mercuric chloride.In a certain time range,with the 10% sodium hypochlorite for sterilization,the explant anti-browning effect was better when the sanitizing time was longer.The optimal hormone collocation for anti-browning was 6-BA and NAA.Among 3 anti-browning agents,the 1 500 mg/L Vc was in the most anti-browning effect.[Conclusion] The best effect of preventing explant from browning was to select the terminal bud without differentiation as explant,0.1% mercuric chloride as disinfectant,MS as basic medium with 6-BA 1.0 mg/L and NAA 0.1 mg/L for tissue culture.