Study on the Browning-inhibition of Longan in Tissue Culture

[Objective] The aim was to seek for the effective method of inhibiting the browning of Longan explants in tissue culture. [Method] With stem segments of tender Longan shoot as material, many methods of resisting browning were used to screen out the method with best inhibition effect on the browning of Longan explants in tissue culture. [Result] The inhibition effect on the browning of Longan explants pretreated with 6.00 g/LPVP solution was best. When the 6-BA was 1.92-1.93 mg/L, KT was 0.88-1.93 mg/L and NAA was 0.05-0.06 mg/L, the browning degree of explants was lightest. The inhibition effect of medium with 2.0 g/L PVP and active carbon on the browning of explants was best. [Conclusion] The combination of medium MS+NAA 0.05-0.06 mg/L+6-BA 1.92-1.93 mg/L+KT 0.88-1.93 mg/L+PVP 2.00 g/L and pretreatment with 6.00 g/L PVP solution had the best inhibition effect on the browning of Longan explants in tissue culture.