Effect of different culture conditions on browning rate of explants in tissue culture of tea trees.

The sterilized time and cutting pattern of explants,interval time for transferring another bottle,medium types,different hormone combination,anti-browning agents and absorption agents were studied to reduce the browning rate of explants in tissue culture of tea tree.The results showed that the browning rate could reduce from 86 % to 41 % when the explants with 2/5 leaf and a axillary bud were sterilized by 0.1 % mercury solution for 7-8 min,the explants were transferred into another bottle every 3d and the treated explants were cultured on 1/2MS +BA 2 mg/L+NAA 0.1 mg/L+Vc 1.0 g/L+AC 1.0 g/L.