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2004•Unpublished venueRequires access

Determination of Icariin in Guilubushen capsules by HPLC

Jian Qiang Zhong

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Abstract

Objective To develop the assay method of icariin in Guilubushen Capsules. Methods HPLC method was used for determination of icariin in Guilubushen Capsules with C 18 column. The mobile phase consisted of water-acetonitrile -tetrahydrofuran-glacial acetic acid (755∶230∶10∶5) and detection wavelength was at 270 nm. Results the average recovery was 98.19% and RSD was 1.2%. Conclusion The method is simple, accurate and can be used in icariin assay of Guilubushen Capsules.

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What this paper is about

Objective To develop the assay method of icariin in Guilubushen Capsules. Methods HPLC method was used for determination of icariin in Guilubushen Capsules with C 18 column. The mobile phase consisted of water-acetonitrile -tetrahydrofuran-glacial acetic acid (755∶230∶10∶5) and detection wavelength was at 270 nm. Results the average recovery was 98.19% and RSD was 1.2%. Conclusion The method is simple, accurate and can be used in icariin assay of Guilubushen Capsules.

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Available abstract

Objective To develop the assay method of icariin in Guilubushen Capsules. Methods HPLC method was used for determination of icariin in Guilubushen Capsules with C 18 column. The mobile phase consisted of water-acetonitrile -tetrahydrofuran-glacial acetic acid (755∶230∶10∶5) and detection wavelength was at 270 nm. Results the average recovery was 98.19% and RSD was 1.2%. Conclusion The method is simple, accurate and can be used in icariin assay of Guilubushen Capsules.

Key concepts: Icariin, Chromatography, Acetic acid, High-performance liquid chromatography, Chemistry, Tetrahydrofuran, Extraction (chemistry), Solvent

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