Study on the Technology of Tissue Culture on Smilax china L.

[Objective] The aim of the research was to develop and utilize Smilax china L.better.[Method] With tender stems of S.china as explants,MS,1/2 MS and 1/4 MS were taken as basic medium to study the effects of different plant hormone combinations on the tissue culture and rapid propagation of S.china.[Result] The optimum disinfection method of explants was disinfecting with 0.1% mercuric chloride for 9 min.Medium 1/2 MS and 1/4 MS were more favorable for the bud induction,propagation and rooting of S. china.6-BA played a key effect on the propagation culture of bud clumping.The optimum initial medium was 1/2 MS+6-BA 2.0 mg/L+IBA 0.1 mg/L+NAA 0.2 mg/L.The optimum propagation medium was 1/2 MS+6-BA 3.0 mg/L+IBA 0.2 mg/L+NAA 0.2 mg/L.The optimum rooting medium was 1/4 MS+IBA 0.1 mg/L+NAA 1.5 mg/L+activated carbon 1 000 mg/L,with the rooting rate being highest(75%).[Conclusion] The tissue culture and propagation system was established and the propagation coefficient of S.china was increased in this research.