Study on Tissue Culture Technology of Begonia elalior

[Objective]The objective of this study was to develop an efficient system for the regeneration of Begonia elalior by investigating the factors influencing callus and shoot induction. [Method] Leaves,petioles and stems of Begonia elalior were used as explants for adventitious bud induction,subculture and rooting. [Result] The optimum medium for the differentiation and elongation for leaves and petioles or stems was MS + 6-BA 1. 0 mg / L + NAA 0. 2 mg / L + adenine 8 mg / L,MS + 6-BA 0. 5 mg / L + NAA 0. 2 mg / L + adenine 8 mg / L,MS + 6-BA 0. 5mg / L + NAA 0. 4 mg / L + 2,4-D 0. 1 mg / L respectively; The optimum medium for the secondary and the rooting culture was MS + 6-BA 1. 0mg / L + IBA 0. 2 mg / L and 1 /2MS + IBA 0. 1 mg / L + NAA 0. 1 mg / L,respectively. [Conclusion] The rapid propagation system of Begonia elalior was established in this study,which could provide theoretical evidence for the rapid propagation and genetic transformation of Begonia elalior.