Establishment of Tissue Culture and Rapid Propagation of Anthurium andraeanurn

The effects of different basic medium and the combination of growth regulators on the adventitious bud inducement,proliferation and rooting conditions were studied to find out the best medium formulation of Anthurium andraeanurn with Arizona,Amigo,Atlanta and SH Red as materials.The results showed that the appropriate sterilization time of adult leaves,petioles,stems and bracts was 15 minutes using mercuric chloride,while 10 minutes for leaves;the best medium of callus inducement was 1/3 MS(NH4 NO3 200 mg/L) + 6-BA 0.5 mg/L +2,4-D 0.05 mg/L + agar 5 g/L + sucrose 30 g/L and the rate of callus inducement was 100%.The best adventitious bud differentiation medium was 1/2 MS+6-BA 1mg/L+agar 5 g/L+sucrose 30 g/L.The best rooting medium was 1/2 MS+IBA 0.05 mg/L+agar 5 g/L+sucrose 30 g/L.The experimental results will provide reference for large-scale production of Anthurium andraeanurn.