Tissue Culture and Rapid Propagation of Anthurium andraeanum
Liu Cun-ping
Abstract
Liu Cun-ping
Abstract
Healthy Anthurium andraeanum seedlings planted in pot were used as materials of tissue culture.The results showed that:High induction rate was on the MS with BA 1 mg/L and NAA 0.2 mg/L and sugar 30 g/L.Callus tissue and bud-seedling multiplication rate were the highest on the 1/2MS with BA 2 mg/L+2.4-D 0.5 mg/L+coconut liquid 100 mL/L+sucrose 30 g/L;The optimum medium for strong seedling was MS+BA 0.5 mg/L+IBA 0.05 mg/L+coconut liquid 100 mL/L+sucrose 30 g/L.The optimum medium for rooting was 1/2MS+NAA 0.3 mg/L+sucrose 20 g/L.The test tube shoots rooting rate reached above 95%.The shoot with 3-4 roots will be transplanted.
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Healthy Anthurium andraeanum seedlings planted in pot were used as materials of tissue culture.The results showed that:High induction rate was on the MS with BA 1 mg/L and NAA 0.2 mg/L and sugar 30 g/L.Callus tissue and bud-seedling multiplication rate were the highest on the 1/2MS with BA 2 mg/L+2.4-D 0.5 mg/L+coconut liquid 100 mL/L+sucrose 30 g/L;The optimum medium for strong seedling was MS+BA 0.5 mg/L+IBA 0.05 mg/L+coconut liquid 100 mL/L+sucrose 30 g/L.The optimum medium for rooting was 1/2MS+NAA 0.3 mg/L+sucrose 20 g/L.The test tube shoots rooting rate reached above 95%.The shoot with 3-4 roots will be transplanted.
Key concepts: Sucrose, Seedling, Anthurium, Shoot, Tissue culture, Callus, Araceae, Horticulture