2007•Zhongguo yaoke daxue xuebaoRequires access
Enantiomeric separation of citalopram by HPLC using α_1-acid glycoprotein chiral stationary phase
Wenying Liu
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Abstract
Aim:To develop a chiral separation method for enantiomers of citalopram by HPLC using α1-acid glycoprotein(α1-AGP) chiral stationary phase.Methods:Influences of the pH value of the mobile phase,the kind and concentration of the buffer solution,organic modifiers,the flow rate and the column temperature on the enantiomeric separation of citalopram using α1-AGP chiral stationary phase were investigated.Results:Citalopram enantiomers were separated on an α1-AGP chiral stationary phase column using water as the mobile phase consisting of 50 mmol/L NH4Ac buffer(pH =4.3) at a flow rate of 0.8 mL/min.Conclusion:The pH value of the mobile phase and the concentration of the buffer solution are very important parameters in the optimization of the enantiomeric separation of the cationic racemates.Higher column efficiency can be achieved with lower flow rate.