Enantiomeric Separation of Pantoprazole by HPLC Using α_1 - Acid Glycoprotein Chiral Stationary Phase

Objective:To develop a chiral separation method for the enantiomers of pantoprazole. Method:Influences of the pH value of the mobile phase, the organic modifiers, the flow rate and the column temperature on the enantiomeric separation of pantoprazole using α1 - acid glycoprotein chiral stationary phase were investigated. Pantoprazole enantiomers were separated on a Chiral -AGP column (150 mm ×4. 6 mm, 5μm)with a mobile phase consisted of 10 mmol· L-1 NH4OAc ( pH 5. 5 ) - acetonitrile (93: 7) at a flow rate of 0. 9 mL· min-1. Results: Pantoprazole enantiomers were separated on an α1 - acid glycoprotein chiral stationary phase and the resolution was 1. 77. The values of enantiomeric excess of pantoprazole were determined under optimized conditions. Conclusion: The pH value of the mobile phase, the organic modifer concentration, the flow rate and the temperature of column were very important when optimizing the enantiomeric separation of pantoprazole.