2012Shandong Agricultural SciencesRequires access

Toxicity Test of Ten Fungicides to Phytophthora infestans in Laboratory

MA Shi-zhong

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Abstract

The toxicity of 10 fungicides against Phytophthora infestans was detected by the method of mycelium growth inhibition.The results showed that 50% dimethomorph WP had the best control effect with EC50 and EC90 as 1.81 μg/ml and 10.63 μg/ml respectively,and the regression equation was Y=4.57272+1.66462X.The followed fungicides were 250 g/L azoxystrobin SC and 75% chlorothalonil WP with the EC50 as 2.70 μg/ml and 2.54 μg/ml,and the EC90 as 1.08×107 μg/ml and 10 708.95 μg/ml respectively.But with the increase of dosage,the control effects of the two fungicides did not increase significantly,and their EC90 value were higher.The control effects of 58% metalaxyl·mancozeb WP and 70% metiram WDP were relatively higher with the EC50 value as 6.09 μg/ml and 8.75 μg/ml,and the EC90 as 69.98 μg/ml and 60.19 μg/ml respectively.Their regression equations were Y=4.05225+1.20831X and Y=3.55894+1.52997X respectively.25% carbendazim WP also could inhibit Phytophthora infestans.Although the extended area of hypha was great,the hypha grew weak and thin.

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The toxicity of 10 fungicides against Phytophthora infestans was detected by the method of mycelium growth inhibition.The results showed that 50% dimethomorph WP had the best control effect with EC50 and EC90 as 1.81 μg/ml and 10.63 μg/ml respectively,and the regression equation was Y=4.57272+1.66462X.The followed fungicides were 250 g/L azoxystrobin SC and 75% chlorothalonil WP with the EC50 as 2.70 μg/ml and 2.54 μg/ml,and the EC90 as 1.08×107 μg/ml and 10 708.95 μg/ml respectively.But with the increase of dosage,the control effects of the two fungicides did not increase significantly,and their EC90 value were higher.The control effects of 58% metalaxyl·mancozeb WP and 70% metiram WDP were relatively higher with the EC50 value as 6.09 μg/ml and 8.75 μg/ml,and the EC90 as 69.98 μg/ml and 60.19 μg/ml respectively.Their regression equations were Y=4.05225+1.20831X and Y=3.55894+1.52997X respectively.25% carbendazim WP also could inhibit Phytophthora infestans.Although the extended area of hypha was great,the hypha grew weak and thin.

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Available abstract

The toxicity of 10 fungicides against Phytophthora infestans was detected by the method of mycelium growth inhibition.The results showed that 50% dimethomorph WP had the best control effect with EC50 and EC90 as 1.81 μg/ml and 10.63 μg/ml respectively,and the regression equation was Y=4.57272+1.66462X.The followed fungicides were 250 g/L azoxystrobin SC and 75% chlorothalonil WP with the EC50 as 2.70 μg/ml and 2.54 μg/ml,and the EC90 as 1.08×107 μg/ml and 10 708.95 μg/ml respectively.But with the increase of dosage,the control effects of the two fungicides did not increase significantly,and their EC90 value were higher.The control effects of 58% metalaxyl·mancozeb WP and 70% metiram WDP were relatively higher with the EC50 value as 6.09 μg/ml and 8.75 μg/ml,and the EC90 as 69.98 μg/ml and 60.19 μg/ml respectively.Their regression equations were Y=4.05225+1.20831X and Y=3.55894+1.52997X respectively.25% carbendazim WP also could inhibit Phytophthora infestans.Although the extended area of hypha was great,the hypha grew weak and thin.

Key concepts: Fungicide, EC50, Carbendazim, Phytophthora infestans, Mancozeb, Mycelium, Horticulture, Hypha

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