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Visible Spectrophotometric Determination of Total Flavonoids in Capparis spinosa L.Buds

Hongjin Bai

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Abstract

Different extraction methods were coupled with visible spectrophotometry to develop a simple and accurate method for quantifying total flavonoids in Capparis spinosa L.buds.The detection wavelength was set at 500 nm.Rutin was used as the standard sample and NaNO3,Al(NO3)3 and NaOH was used to compose the coloration system.Results indicated that the methanol reflux method exhibited the highest extraction rate for total flavonoids.A good linear relationship was observed in the range of 0 to 32 mg/L(r = 0.9997) and average recovery rate was 100.3% with a relative standard deviation of 0.98%(n=6).The average content of total flavonoids in the samples from Kuche county,Xinjiang autonomous region was 1.13%.

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What this paper is about

Different extraction methods were coupled with visible spectrophotometry to develop a simple and accurate method for quantifying total flavonoids in Capparis spinosa L.buds.The detection wavelength was set at 500 nm.Rutin was used as the standard sample and NaNO3,Al(NO3)3 and NaOH was used to compose the coloration system.Results indicated that the methanol reflux method exhibited the highest extraction rate for total flavonoids.A good linear relationship was observed in the range of 0 to 32 mg/L(r = 0.9997) and average recovery rate was 100.3% with a relative standard deviation of 0.98%(n=6).The average content of total flavonoids in the samples from Kuche county,Xinjiang autonomous region was 1.13%.

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Available abstract

Different extraction methods were coupled with visible spectrophotometry to develop a simple and accurate method for quantifying total flavonoids in Capparis spinosa L.buds.The detection wavelength was set at 500 nm.Rutin was used as the standard sample and NaNO3,Al(NO3)3 and NaOH was used to compose the coloration system.Results indicated that the methanol reflux method exhibited the highest extraction rate for total flavonoids.A good linear relationship was observed in the range of 0 to 32 mg/L(r = 0.9997) and average recovery rate was 100.3% with a relative standard deviation of 0.98%(n=6).The average content of total flavonoids in the samples from Kuche county,Xinjiang autonomous region was 1.13%.

Key concepts: Rutin, Capparis spinosa, Relative standard deviation, Extraction (chemistry), Recovery rate, Chemistry, Chromatography, Linear relationship

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Visible Spectrophotometric Determination of Total Flavonoids in Capparis spinosa L.Buds — Research Paper | ScholarLens