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2008•Chinese Journal of Ethnomedicine and EthnopharmacyRequires access

Determination of adenosine in fruit of Capparis spinosa L. with RP-HPLC

Yun Sun

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Abstract

Objective To establish a RP-HPLC method of determination of adenosine in fruit of Capparis spinosa L. Methods: ODS column was Polaris (C18-A 250mm×4.6mm,5μm,AB17),and the mobile phase consissted of methanol-water(15∶85).The detecting wavelength was 260nm and The flow rate was 1.0mL/min.The column temperature was 30℃. Result:The regression equation was Y=206997300X+113157.8(r=0.9991 n=5),the average recovery was 104.6%,RSD=4.51%,the content of adenosine was 36.61mg/Kg(RSD=2.89%,n=5).Conclusion: The method was simple,convenient and accurate for determination of adenosine in fruit of Capparis spinosa L.

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Objective To establish a RP-HPLC method of determination of adenosine in fruit of Capparis spinosa L. Methods: ODS column was Polaris (C18-A 250mm×4.6mm,5μm,AB17),and the mobile phase consissted of methanol-water(15∶85).The detecting wavelength was 260nm and The flow rate was 1.0mL/min.The column temperature was 30℃. Result:The regression equation was Y=206997300X+113157.8(r=0.9991 n=5),the average recovery was 104.6%,RSD=4.51%,the content of adenosine was 36.61mg/Kg(RSD=2.89%,n=5).Conclusion: The method was simple,convenient and accurate for determination of adenosine in fruit of Capparis spinosa L.

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Available abstract

Objective To establish a RP-HPLC method of determination of adenosine in fruit of Capparis spinosa L. Methods: ODS column was Polaris (C18-A 250mm×4.6mm,5μm,AB17),and the mobile phase consissted of methanol-water(15∶85).The detecting wavelength was 260nm and The flow rate was 1.0mL/min.The column temperature was 30℃. Result:The regression equation was Y=206997300X+113157.8(r=0.9991 n=5),the average recovery was 104.6%,RSD=4.51%,the content of adenosine was 36.61mg/Kg(RSD=2.89%,n=5).Conclusion: The method was simple,convenient and accurate for determination of adenosine in fruit of Capparis spinosa L.

Key concepts: Chromatography, Capparis spinosa, Adenosine, High-performance liquid chromatography, Chemistry, Traditional medicine, Medicine, Biochemistry

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