Experimental study of inhibitory effect of arsenic trioxide on human SHG-44 glioma cells in Vitro

Objective To explore the mechanism of proliferative inhibition and apoptosis of arsenic trioxide(As2O3) on human SHG-44 glioma cells.Methods MTT colorimetric method was applied to detect the proliferative inhibition of As2O3 on glioma cells.The morphologic changes of SHG-44 glioma cells were observed by inverted microscope and fluorescence microscope.Cell apoptosis and interference on cell cycle were detected by FCM.Results As2O3 could inhibit the proliferation of SHG-44 glioma cells obviously and the inhibitory effect was a dose-dependent and time-dependent tendency.We observed characteristic changes of cell apoptosis and the cells in treatment group grew with low density.FCM shows that apoptosis cells of the treatment groups were obviously higher than that of control group(P0.05) in a dose-dependent and time dependent tendency.At the same time,As2O3 could block the cells in S phase and interfer the cell cycle progression.Conclusion As2O3 could inhibit the proliferation of SHG-44 glioma cells obviously,induce SHG-44 glioma cell apoptosis and interfere the cell cycle progression.