Fermentation Conditions for Antagonistic Endophytic Bacteria Against Ralstonia solanacearum

The effects of fermentation duration and temperature,bacterium age,inoculum amount,rotating speed of bottle shaking and dissolved oxygen quantity on bacterium amount,and the relationship between fermentation conditions and the amount of Xiang 2-3,a strain against Ralstonia solanacearum,were studied,and the optimum fermentation conditions were determined by orthogonal test.The results indicated that the optimum fermentation conditions for Xiang 2-3 were: fermenting for 24h at 28℃,bacterium age 18h to 22h,inoculum amount 4%,rotating speed 210r/min,and dissolved oxygen 100mL/150 mL.