Submerged Culture Optimization for Biomass and Triterpenoids Production by Inonotus obliquus Using Response Surface Methodology

This thesis uses one-factor-one-time method and finds that glucose and mixed nitrogen sources are the best carbon and nitrogen sources.With the fractional factorial experiment design,it screens the significant effect of glucose,peptone,CaCl2 and yeast powder on triterpenoids biosynthesis.Ultimately,the authors employ the response surface methodology to optimize the medium composition for biomass and triterpenoids production by I.obliquus in shake flask culture.The optimal medium composition is: glucose 58.9 g/L,peptone 2.9 g/L,CaCl2 0.5 g/L,yeast powder 1.2 g/L,KH2PO4 1.0 g/L and MgSO4 0.2 g/L.Under optimal conditions,the authors significantly enhance the triterpenoids production and the mycelial biomass from I.obliquus and the triterpenoids production reaches up to 57.1 mg/g,representing an increase of 36.6% and 57.3% compared with that using the basal medium(41.8 mg/g) and natural sclerotia(36.3 mg/g),respectively.