Molecular Cloning and Prokaryotic Expression of Ubiquitin Ligase Rbx1 from Huangzaosi

Molecular cloning and sequence analysis of Huangzaosi Rbx1 were carried out based on RT-PCR method and gene cloning technique.At the same time,the prokaryotic expression system of Huangzaosi Rbx1 was constructed and the recombinant protein with a His-tag was successfully expressed.Furthermore,western blot with His antibody was used to identify the recombinant protein.The results showed that the recombinant protein had immune reaction with His antibody,and verified that Rbx1 from Huangzaosi could be successfully expressed in prokaryotic expression system.