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2010•Hubei nongye kexueRequires access

Cloning and Prokaryotic Expression of AtCpNifS from Arabidopsis thaliana

Daqing Chen

Open publisher page 1 citations

Abstract

The whole coding domain sequence(CDS) of AtCpNifS was isolated from Arabidopsis thaliana ecotype Columbia.The integrity and accuracy of cloned sequence was confirmed by sequencing and aligning.On this basis,the prokaryotic expression vector(BL21-pET28-AtCpNifS) with the gene was constructed and the expression protein(AtCpNifS) was separated from the plasmid successfully.

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What this paper is about

The whole coding domain sequence(CDS) of AtCpNifS was isolated from Arabidopsis thaliana ecotype Columbia.The integrity and accuracy of cloned sequence was confirmed by sequencing and aligning.On this basis,the prokaryotic expression vector(BL21-pET28-AtCpNifS) with the gene was constructed and the expression protein(AtCpNifS) was separated from the plasmid successfully.

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Available abstract

The whole coding domain sequence(CDS) of AtCpNifS was isolated from Arabidopsis thaliana ecotype Columbia.The integrity and accuracy of cloned sequence was confirmed by sequencing and aligning.On this basis,the prokaryotic expression vector(BL21-pET28-AtCpNifS) with the gene was constructed and the expression protein(AtCpNifS) was separated from the plasmid successfully.

Key concepts: Arabidopsis thaliana, Cloning (programming), Plasmid, Biology, Gene, Ecotype, Arabidopsis, Coding region

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