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Studies on Genetic Diversity of 48 Tobacco Varieties by RAPD Analysis

Yin Han-qi

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Abstract

RAPD molecular-marker technique was used to detect the genetic diversity among 48 tobacco germplasms. The genomic DNA of 48 tobacco germplasms was tested with RAPD using 28 arbitrary 10-mer primers which were screened from 200 arbitrary 10-mer primers. The results showed that the 184 RAPD bands were obtained altogether with 86 bands of polymorphic ones. The percentage of polymorphic bands was 46.7%.The variation range of genetic distances was 0 to 7.81. These varieties were divided into N. tobacum group and N. rustica group by UPGMA cluster analysis based on RAPD molecular-markers data. N. tobacum group could be divided into 4 sub groups.

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What this paper is about

RAPD molecular-marker technique was used to detect the genetic diversity among 48 tobacco germplasms. The genomic DNA of 48 tobacco germplasms was tested with RAPD using 28 arbitrary 10-mer primers which were screened from 200 arbitrary 10-mer primers. The results showed that the 184 RAPD bands were obtained altogether with 86 bands of polymorphic ones. The percentage of polymorphic bands was 46.7%.The variation range of genetic distances was 0 to 7.81. These varieties were divided into N. tobacum group and N. rustica group by UPGMA cluster analysis based on RAPD molecular-markers data. N. tobacum group could be divided into 4 sub groups.

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Available abstract

RAPD molecular-marker technique was used to detect the genetic diversity among 48 tobacco germplasms. The genomic DNA of 48 tobacco germplasms was tested with RAPD using 28 arbitrary 10-mer primers which were screened from 200 arbitrary 10-mer primers. The results showed that the 184 RAPD bands were obtained altogether with 86 bands of polymorphic ones. The percentage of polymorphic bands was 46.7%.The variation range of genetic distances was 0 to 7.81. These varieties were divided into N. tobacum group and N. rustica group by UPGMA cluster analysis based on RAPD molecular-markers data. N. tobacum group could be divided into 4 sub groups.

Key concepts: RAPD, UPGMA, Genetic diversity, Biology, Genetics, Genetic variation, Veterinary medicine, Gene

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