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2006•Journal of Pharmaceutical and Biomedical SciencesRequires access

Analysis of Genetic Diversity in Tobacco Germplasm by RAPDs and AFLPs

Yang You-cai

Open publisher page 1 citations

Abstract

The genomic DNA of 48 tobacco germplasms were tested with RAPD using 28 arbitrary 10-mer primers which were screened from 200 primers. The 86 polymorphic bands of 184 RAPD bands were obtained. The percentage of polymorphic bands was 46.7%.All the DNAs were amplified by 4 AFLP selective primers. The 174 are polymorphic bands of 321 AFLP bands were obtained. The percentage of polymorphic bands was 54.2%.UPGMA cluster analysis based on two molecular markers data divided 48 varieties into two groups: Nicotiana tobacum and N. rustica groups. N.t obacum group could be classified into four subgroups. The genetic distance of 48 cultivars was between 1.4～11.0 and was basically according with theoretic expectation case. The results showed that the AFLP approach was more efficient in generating accurate information on genetic background, relationship and evolution of tobacco germplasms than that of RAPD method.

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What this paper is about

The genomic DNA of 48 tobacco germplasms were tested with RAPD using 28 arbitrary 10-mer primers which were screened from 200 primers. The 86 polymorphic bands of 184 RAPD bands were obtained. The percentage of polymorphic bands was 46.7%.All the DNAs were amplified by 4 AFLP selective primers. The 174 are polymorphic bands of 321 AFLP bands were obtained. The percentage of polymorphic bands was 54.2%.UPGMA cluster analysis based on two molecular markers data divided 48 varieties into two groups: Nicotiana tobacum and N. rustica groups. N.t obacum group could be classified into four subgroups. The genetic distance of 48 cultivars was between 1.4～11.0 and was basically according with theoretic expectation case. The results showed that the AFLP approach was more efficient in generating accurate information on genetic background, relationship and evolution of tobacco germplasms than that of RAPD method.

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Available abstract

The genomic DNA of 48 tobacco germplasms were tested with RAPD using 28 arbitrary 10-mer primers which were screened from 200 primers. The 86 polymorphic bands of 184 RAPD bands were obtained. The percentage of polymorphic bands was 46.7%.All the DNAs were amplified by 4 AFLP selective primers. The 174 are polymorphic bands of 321 AFLP bands were obtained. The percentage of polymorphic bands was 54.2%.UPGMA cluster analysis based on two molecular markers data divided 48 varieties into two groups: Nicotiana tobacum and N. rustica groups. N.t obacum group could be classified into four subgroups. The genetic distance of 48 cultivars was between 1.4～11.0 and was basically according with theoretic expectation case. The results showed that the AFLP approach was more efficient in generating accurate information on genetic background, relationship and evolution of tobacco germplasms than that of RAPD method.

Key concepts: RAPD, UPGMA, Biology, Germplasm, Amplified fragment length polymorphism, Genetic diversity, Genetics, Genetic distance

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