ScholarLens Back to search
2008•Shiyong kouqiang yixue zazhiRequires access

Effects of EMPs on proliferation and mineralization of human BMSCs

Xie Yufen

Open publisher page 0 citations

Abstract

Objective:To investigate the effects of enamel matrix proteins(EMPs) on cell cycle and alkaline phosphate (ALP) activity of human bone marrow stromal cells(BMSCs). Methods:Human BMSCs were cultured in DMEM medium with 10% fetal bovine serum(FBS) in vitro. EMPs was added into medium at a concentration of 200 μg /ml as experimental group. BMSCs were cultured without EMPs as control group. Cell cycle was evaluated by flow cytometry. ALP activity was also detected by ALP detection kit. Results:EMPs at a concentration of 200 μg/ml could enhance ALP activity and affect cell cycle of BMSCs. Cell number in S stage was 19.48% in EMPs group,and 9.89% in control group.Conclusion:EMPs can promote ALP activity of human BMSCs,and affect cell cycle of BMSCs. EMPs may act as a kind of promotive factor for BMSCs.

About this research paper

What this paper is about

Objective:To investigate the effects of enamel matrix proteins(EMPs) on cell cycle and alkaline phosphate (ALP) activity of human bone marrow stromal cells(BMSCs). Methods:Human BMSCs were cultured in DMEM medium with 10% fetal bovine serum(FBS) in vitro. EMPs was added into medium at a concentration of 200 μg /ml as experimental group. BMSCs were cultured without EMPs as control group. Cell cycle was evaluated by flow cytometry. ALP activity was also detected by ALP detection kit. Results:EMPs at a concentration of 200 μg/ml could enhance ALP activity and affect cell cycle of BMSCs. Cell number in S stage was 19.48% in EMPs group,and 9.89% in control group.Conclusion:EMPs can promote ALP activity of human BMSCs,and affect cell cycle of BMSCs. EMPs may act as a kind of promotive factor for BMSCs.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To investigate the effects of enamel matrix proteins(EMPs) on cell cycle and alkaline phosphate (ALP) activity of human bone marrow stromal cells(BMSCs). Methods:Human BMSCs were cultured in DMEM medium with 10% fetal bovine serum(FBS) in vitro. EMPs was added into medium at a concentration of 200 μg /ml as experimental group. BMSCs were cultured without EMPs as control group. Cell cycle was evaluated by flow cytometry. ALP activity was also detected by ALP detection kit. Results:EMPs at a concentration of 200 μg/ml could enhance ALP activity and affect cell cycle of BMSCs. Cell number in S stage was 19.48% in EMPs group,and 9.89% in control group.Conclusion:EMPs can promote ALP activity of human BMSCs,and affect cell cycle of BMSCs. EMPs may act as a kind of promotive factor for BMSCs.

Key concepts: Stromal cell, Chemistry, Cell cycle, Flow cytometry, Andrology, Cell counting, Cell, Human bone

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effects of EMPs on proliferation and mineralization of human BMSCs — Research Paper | ScholarLens