Impact on early bone metabolism and E_2 expression of ovariectomized rats using puerarin combined with sport

Objective To observe the impact on early bone metabolism and serum E2 expression of ovariectomized rats(OCR) using puerarin combined with moderate intensity exercise.Methods Forty-eight 12-week female SD rats were randomly divided into 6 groups(A-F,8/group).Under 0.3% pentobarbital sodium(30 mg/kg) anesthesia conditions,rats of group B to F were opened abdominal and removed bilateral ovary and rats of group A were pure opened abdominal without removing bilateral ovary.One week after operation,rats of group A and B were administered nothing;rats of group C were administered estrogen 200 μg/(kg·d),intramuscular injection for 12 weeks;rats of group D were administered puerarin 50 mg/(kg·d),intraperitoneal injection for 12 weeks;rats of group E were intervened with moderate intensity exercise;rats of group F were treated with puerarin combined with sport.Conventional breeding was used to all of the rats.After treatment for 12 weeks,serum of the rats was collected and spined,and the content of Ca and P were analyzed using electrode technique,the level of E2 was analyzed by using radioimmunity method.Bone struction of right upper tibia were examined by using HE staining.Results The concentration of Ca in group B was higher than group C,D and F which had significant difference(P0.05) and the concentration of ALP in group B was lower than group C and F(P0.05).The ultrastructure of rats tibia were observed through HE staining.The number of bone trabeculae and thickness were decreased significantly in group B,and the bone structure changed from strong density cellular structure to sparse loose irregular appearance beehive structure.While in group F,bone trabeculae had good continuity,compact structure and less fat empty bubble.Conclusion Puerarin combined with moderate intensity exercise can promote bone-formation and has similar role to single high-dose E2.