2013•Zhongguo fuyou baojianRequires access
Effect of puerarin combined with estradiol on bone tissue and serum calcium,phosphorus,and alkaline phosphatase in ovariectomized rats
Jin Wang
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Abstract
Objective:To understand the effect of puerarin combined with estradiol on bone tissue and serum calcium,phosphorus,and alkaline phosphatase in ovariectomized rats,and provide experimental basis for treating postmenopausal osteoporosis with traditional Chinese medicine combined with Western medicine.Methods:One hundred and twenty 5-month-old female rats were selected and divided into five experimental groups,24 rats in each group: sham-operation group,ovariectomized model group,puerarin group(the rats were treated with subcutaneous injection of puerarin,50 mg/kg,once a day),estradiol group(the rats were treated with subcutaneous injection of estradiol,200 μg/kg,twice a week),low-dose puerarin combined with estradiol group(combined therapy group)(the rats were treated with subcutaneous injection of estradiol,100 μg/kg,twice a week;subcutaneous injection of puerarin,25 mg/kg,once a day).At 4,8,12,and 20 weeks after start of the experiment,6 rats were randomly selected,femur smears were prepared to observe bone tissue;the blood samples were obtained to detect serum levels of calcium,phosphorus,and alkaline phosphatase;the data were analyzed statistically.Results:The pathological changes of osteoporosis were observed at 4,8,12,and 20 weeks in ovariectomized model group,the serum levels of calcium and phosphorus in ovariectomized model group were statistically significantly lower than those in sham-operation group(P﹤0.01);the serum levels of alkaline phosphatase at 4,8,12,and 20 weeks in ovariectomized model group were statistically significantly higher than those in sham-operation group(P﹤0.01).There was no statistically significant difference in bone tissue,serum levels of calcium,phosphorus,and alkaline phosphatase at 4,8,12,and 20 weeks between three treatment groups and sham-operation group(P﹥0.05).Bone tissue,serum levels of calcium,phosphorus,and alkaline phosphatase in combined therapy group basically returned to normal(P﹥0.05),compared with puerarin group and estradiol group,there was no statistically significant difference(P﹥0.05).Conclusion:The curative effect of low-dose estradiol combined with puerarin for treatment of osteoporosis in ovariectomized rats is similar to curative effects of relatively high-dose puerarin or estradiol.