THE STUDY OF TIME-RESOLVED FLUO RESCENCE IMMUNOUSSAY IN DECECTION OF SENCM MARKERS OF HEPATITIS B VIRUS

Objective: To study the clinical application value of time-resolved fluorescence immunoassay( TRFIA) for detection of serum markers of hepatitis B virus. Methods: Using enzyme immunoassay( ELISA) and time-resolved fluorescence immunoassay respectively in 185 serum specimens,hepatitis B markers were qualitatively and quantitatively detected and compared,analysis of the results. Results: Five makers of hepatitis B was measured with good accuracy and repeatability by TRFIA. There was no remarkable significance between ELISA and TRFIA in detecting HB s Ag、anti-HB s 、HB e Ag ang anti-HB e( P0. 05). But there was significant difference between ELISA and TRFIA in detecting antiHB c( P0. 05). In each 10 samples of high and medium concentrations of HB s Ag,all were positive by TRFIA and ELISA. However,the detection rate with TRFIA assay was higher than that with ELISA assay in 10 samples of low concentration. There were nine modes in two methods,positive example was dose to each other in six modes,there were difference in three modes. Conclusions: Time-resolved fluorescence immunoassay for detection of serum markers of hepatitis B virus had good sensitivity and good specificity and it is helpful to estimate the effect.