2013•Hainan yixueRequires access
Clinical application of time-resolved fluoroimmunoassay for detecting hepatitis B virus surface antigen
Wang Xion
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Abstract
Objective To investigate the clinical application of time-resolved fluoroimmunoassay(TRFIA) in quantitative measurement of hepatitis B virus surface antigen.Methods Quantitative and qualitative analysis of 237 serum samples were detected by TRFIA and ELISA,respectively.Twenty samples of high,medium and low concentration of HBsAg were taken respectively for redetermination by the two methods.Results Of the 237 samples,99 were positive for HBsAg with TRFIA assay,and 91 were positive for HBsAg with ELISA assay.There was no statistically significant difference between the two methods(P0.05).The 20 samples of high or medium concentration of HBsAg were all positive by both TRFIA and ELISA,with no statistically significant difference between two methods(P0.05).However,the detection rate with TRFIA assay was significantly higher than that with ELISA assay in the 20 samples of low concentration(P0.01).Conclusion TRFIA assay is more sensitive in quantitative detection of hepatitis B virus surface antigen.Moreover,TRFIA assay has more advantages than ELISA in detection of low concentration samples.We believe that TRFIA has a higher application value.