Distribution of-260 C/T single nucleotide polymorphisms (SNP) in the promoter region of CD14 gene and its significance for coronary heart disease and plasma lipids levels between the Han and the Uyghur people in Xinjiang

Objective To investigate the distribution of CD14 promotergene C(-260)→T polymorphism between the Han and Uyghur population in xinjiang, analyze its significance for coronary heart disease and plasma lipids levels between the Han and the Uyghur people in Xinjiang. Methods Genotypes of CD14 were typed in 390 included subjects by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP);Simultaneous determination of blood lipid level. As a gold standard, selected coronary angiography was performed in all patients. Results The frequency of genotype CC,CT and TT in CHD group and control group was respectively 20.33%,52.70%,26.97% and 37.58%,43.63%,18.79%,which of C, T allele was respectively 46.68%,53.32% and 59.40%,40.60 %. No statistically significant difference was found between the two groups. Compared with other ethnics, there exited difference only between Czech population and Chinese population (P0.05). No association was found between the genotypes and plasma lipids levels(P0.05). Conclusion The polymorphism of CD14 C(-260)→T was associated with CHD in xinjiang population. T allele may be a risk factor for CHD. There was no significant difference between the Han and the Uyghur groups. Compared with Czech,Germany, Japan and America population, significant difference was found only between Czech population and Chinese population(P0.05).There was no significant difference between different genotype groups. The pathogenic mechanism of the gene Polymorphism was possibly unrelated to blood lipid metabolism.