Simultaneous determination of baicalin and luteolin in Chrysanthemum morifolium Ramat.using reverse phase high performance liquid chromatography

A reverse phase high performance liquid chromatographic method was developed for simultaneous determing the contents of baicalin and luteolin in Chrysanthemum morifolium Ramat..The sample was extracted by ultrasonic.The mobile phase was phosphate buffer solution(pH 2.6)(A) and methanol(B) with gradient elution(0-3 min,30%A～35%A;3-6 min,35%A～40%A;6-9 min,40%A).The chromatographic analysis was carried out on a purospher star RP-18 column(250 mm×4.6 mm,5 μm).The detection wavelength was set at 327 nm.The sample injection volune was 20 μL.The calibration cure was linear over the range of 4.30-43.00 mg/L and 5.00-10.00 mg/L(r0.999 5) for baicalin and luteolin,respectively.The average recoveries were 98.56% for baicalin and 100.16% for luteolin with the relative standard deviations of 1.68% and 3.20%,respectively.This simple,rapid and accurate method is suitable for the quality control of Chrysanthemum morifolium Ramat.