A Comparative Quality Analysis of Chrysanthemum Morifolium from Five Defferent Production Areas

OBJECTIVE To establish a HPLC method for determination of luteolin and Chlorogenic Acid in chrysanthemum flower from different areas.METHODS ①The Chlorogenic Acid was separated on a column:Dikma Diamonsil C18(200 mm×4.6 mm,5 μm) column;mobile phase:methanol-0.1 mol·L-1.natrium dihydrogen phosphate solution(15.6 g→1 000 mL,pH=2.7)(20:80);the flow rate 1.0 mL·min-1;the column temperature 30℃;the detective wavelength was 328 nm.②The Luteolin was sparated on a Dikma Diamonsil C18(200 mm×4.6 mm,5 μm)column,the mobile phase:methanol-water(55:45),flow rate 1.0 mL·min-1,UV wavelength 353 nm,column temperature 30 ℃.RESULTS ①The Chlorogenic Acid calibration curve showed good linearity in the range of 0.2～1.0 μg(r=0.999 9),the average recovery was 98.25%(RSD=0.71%).②The Luteolin calibration curve showed good linearity in the range of 0.1～1.0 μg(r=0.999 9),the average recovery was 98.99%(RSD=1.03%).CONCLUSION The method is simple,accurate and specific.