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2010•Journal of Anhui Science and Technology UniversityRequires access

Extraction by Ultrasonic and Determination by HPLC of Luteolin from Chrysanthemum morifolium Ramat

Jianfei Wang

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Abstract

The method of extraction by ultrasonic and determination by high performance liquid chromatographic of luteolin from Chrysanthemum morifolium Ramat was established.The chromatographic analysis was carried out on a purospher star RP-18 column(250 mm×4.6 mm,5 μm).The mobile phase was phosphate buffer solution(pH2.6) and methanol with isocratic elution(4060).The flow rate was maintained at 1 mL/min.The detection wavelength was set at 327 nm.The results showed that Luteolin was separated quickly within 10 min.The calibration cure was linear over the range of 0.002~0.05 mg/mL,r=0.9999,the average recovery was 99.22%,RSD was 1.7%.This simple,rapid and accurate method is suitable for the quality control of Chrysanthemum morifolium Ramat.

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What this paper is about

The method of extraction by ultrasonic and determination by high performance liquid chromatographic of luteolin from Chrysanthemum morifolium Ramat was established.The chromatographic analysis was carried out on a purospher star RP-18 column(250 mm×4.6 mm,5 μm).The mobile phase was phosphate buffer solution(pH2.6) and methanol with isocratic elution(4060).The flow rate was maintained at 1 mL/min.The detection wavelength was set at 327 nm.The results showed that Luteolin was separated quickly within 10 min.The calibration cure was linear over the range of 0.002~0.05 mg/mL,r=0.9999,the average recovery was 99.22%,RSD was 1.7%.This simple,rapid and accurate method is suitable for the quality control of Chrysanthemum morifolium Ramat.

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Available abstract

The method of extraction by ultrasonic and determination by high performance liquid chromatographic of luteolin from Chrysanthemum morifolium Ramat was established.The chromatographic analysis was carried out on a purospher star RP-18 column(250 mm×4.6 mm,5 μm).The mobile phase was phosphate buffer solution(pH2.6) and methanol with isocratic elution(4060).The flow rate was maintained at 1 mL/min.The detection wavelength was set at 327 nm.The results showed that Luteolin was separated quickly within 10 min.The calibration cure was linear over the range of 0.002~0.05 mg/mL,r=0.9999,the average recovery was 99.22%,RSD was 1.7%.This simple,rapid and accurate method is suitable for the quality control of Chrysanthemum morifolium Ramat.

Key concepts: Chrysanthemum morifolium, Chromatography, Luteolin, Extraction (chemistry), Chemistry, High-performance liquid chromatography, Elution, Methanol

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Extraction by Ultrasonic and Determination by HPLC of Luteolin from Chrysanthemum morifolium Ramat — Research Paper | ScholarLens