Determination of Rutin and Quercetin in Hypericum Periforatum of Xinjiang by HPLC

An HPLC method was used for the determination of rutin and quercetin in Hypericum perforatum.The rutin and quercetin was separated on a Hyperil ODS(12.5 cm×6.0 mm i.d.,5μm) with a mixture of methanol and 0.4% phosphoric acid as mobile phase.The detection wavelength was 360 nm.The linearity of rutin was found in the range of 0.058～0.580 μg,r=0.999 8,the average recovery was 99.0 %,RSD=0.66 %(n=6) And the linearity of quercetin was 0.014～0.140 μg,r=0.999 9,the average recovery was 95.9 %,RSD=1.60 %((n=6).) This method is reliable,accurate and suitable for the determination of rutin and quercetin.