Effects of vitamin A on cervical cancer cell proliferation and apoptosis

Objective To exploe the effect of vitamin A on cervical cancer cell proliferation and apoptosis.Methods The proliferation inhibition rate of human cervical cancer Caski was analyzed by MTT after treating by 5,7.5,10,15 μg/ml of vitamin A.Cell cycle of Caski,Siha and Hela treated by vitamin a were detected by flow cytometry.Mitochondrial membrane potential was examined by fluorescence microplate reader;Apoptosis of Caski cell treated with vitamin A was detected by deoxynucleotidyl TUNEL.Results Cell proliferation inhibition rates of Caski treated by 5,7.5,10,15 μg/ml vitamin A for 24 h were 32.1%±2.6%,47.3%±1.1%,66.4%±2.1%,70.8%±2.7%,respectively,after 48 h treatment were 38.3%±2.8%,52.6%±3.1%,72.5%±2.4%,80.0%±4.9%.Vitamin A can inhibit cervical cell proliferation in a time and concentration dependent manner(all P0.05).The number of Caski,Siha and Hela cells after vitamin A treatment in G0/G1 and G2/M phase significantly reduced,while S phase cells increased,cells were blocked in the G0 phase,the mitochondria potential of Caski,Siha and Hela cells was 2.21±0.29,2.05±0.35,2.38±0.22,respectively,and pre-treatment was 0.32±0.08,0.24±0.15,0.41±0.06(all P0.05).Conclusions Vitamin A can inhibit cervical cancer cell proliferation and induced apoptosis.