Effect of mifepristone on proliferation of cervical carcinoma Caski cells

Objective:To explore the effect of mifepristone on proliferation of human cervical carcinoma Caski cells and the mechanism,provide a experimental basis for treatment of cervical squamous carcinoma with mifepristone.Methods:Human cervical squamous carcinoma cell line Caski cells were cultured in vitro,then Caski cells were treated with various concentrations of mifepristone,the effect of mifepristone on proliferation of human cervical carcinoma Caski cells was detected by 4 methyl thiazolyl tetrazolium (MTT) assay;the changes of cell cycle was analyzed using flow cytometry;the changes of expression levels and activity of HPV-E6 and p53 in Caski cells were investigated by FITC staining flow cytometry.Results:MTT assay indicated that mifepristone (more than 12.5 mg/L) could suppress the proliferation of Caski cells in vitro,showing a obvious concentration-time trend,1.25 mg/L mifepristone had no significant effect on proliferation of Caski cells (IR5%).The results of flow cytometry indicated that mifepristone more than 12.5 mg/L could inhibit the cell cycle of Caski cells at G1 phase,showing a obvious concentration-time trend.FITC staining flow cytometry indicated that the expression of HPV16-E6 protein decreased and the expression of p53 protein increased in Caski cells treated with mifepristone,showing a obvious concentration dependent trend (P0.05).Conclusion:Large dose of mifepristone (12.5～20.0 mg/L) can suppress the growth of human cervical squamous carcinoma Caski cells significantly,and arrest cell cycle at G1 phase.Mifepristone can suppress the proliferation of Caski cells,the mechanisms are related to down-regulation of HPV16-E6 protein and up-regulation of p53 protein.