Simultaneous determination of muscone and borneol of xingnaojing glucose injection by GC

Aim To establish a GC method for the simultaneous determination of muscone and borneol of xingnaojing glucose injection.Methods DM-17 capillary column(0.32 mm×30 m×0.25 μm,coated with 50% phenyl and 50% methyl-polysiloxan) was adopted and the carrier gas was nitrogen with 2 ml·min-1 of flow rate.The initial column temperature was kept at 70℃ for 2 min,then raised to 200℃ and kept for 10 min at the rate of 10℃·min-1.The detector was FID.Results The correlation coefficient of muscone and borneol were all higher than 0.999.The average recovery were 97.2%,97.0% and the limit of detection were 0.25,0.16 mg·L-1,respectively.Conclusion This method is sensitive,accurate,repeatable and easy to operate for the simultaneous determination of muscone and borneol of xingnaojing glucose injection.