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2011•China Modern MedicineRequires access

Determination of Ginkgolides in teas of Ginkgo biloba by HPLC-ELSD

Ruiming Li

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Abstract

Objective:To establish an RP-HPLC method for quantitative determination of Ginkgolides in teas of Ginkgo biloba.Methods:The evaporation light scattering detector(ELSD) were employed.The analytical column was C18 column(4.6 mm× 250 mm,5 μm).The mobile phase was composed of methanol-tetrahydrofuran-water(25:10:65) at a flow rate of 1.0 ml/min.Results:The logarithm value of sample size had a good linear relationship with the logarithm value of peak area when the sample size of ginkgolide A,B,C and bilobalide was 0.485～9.701 μg,0.460～9.204 μg,0.501～11.899 μg,0.935～18.703 μg respectively.The average recovery was 100.45%(RSD=2.79%),98.29%(RSD=2.90%),98.22%(RSD=2.53%),101.32%(RSD=1.81%) respectively.Conclusion:This method is sensitive,repeatable and suitable to determine the content of total Ginkgolides in teas of Ginkgo biloba.

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Objective:To establish an RP-HPLC method for quantitative determination of Ginkgolides in teas of Ginkgo biloba.Methods:The evaporation light scattering detector(ELSD) were employed.The analytical column was C18 column(4.6 mm× 250 mm,5 μm).The mobile phase was composed of methanol-tetrahydrofuran-water(25:10:65) at a flow rate of 1.0 ml/min.Results:The logarithm value of sample size had a good linear relationship with the logarithm value of peak area when the sample size of ginkgolide A,B,C and bilobalide was 0.485～9.701 μg,0.460～9.204 μg,0.501～11.899 μg,0.935～18.703 μg respectively.The average recovery was 100.45%(RSD=2.79%),98.29%(RSD=2.90%),98.22%(RSD=2.53%),101.32%(RSD=1.81%) respectively.Conclusion:This method is sensitive,repeatable and suitable to determine the content of total Ginkgolides in teas of Ginkgo biloba.

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Available abstract

Objective:To establish an RP-HPLC method for quantitative determination of Ginkgolides in teas of Ginkgo biloba.Methods:The evaporation light scattering detector(ELSD) were employed.The analytical column was C18 column(4.6 mm× 250 mm,5 μm).The mobile phase was composed of methanol-tetrahydrofuran-water(25:10:65) at a flow rate of 1.0 ml/min.Results:The logarithm value of sample size had a good linear relationship with the logarithm value of peak area when the sample size of ginkgolide A,B,C and bilobalide was 0.485～9.701 μg,0.460～9.204 μg,0.501～11.899 μg,0.935～18.703 μg respectively.The average recovery was 100.45%(RSD=2.79%),98.29%(RSD=2.90%),98.22%(RSD=2.53%),101.32%(RSD=1.81%) respectively.Conclusion:This method is sensitive,repeatable and suitable to determine the content of total Ginkgolides in teas of Ginkgo biloba.

Key concepts: Ginkgolides, Ginkgo biloba, Chromatography detector, Chromatography, High-performance liquid chromatography, Medicine, Ginkgoales, Ginkgo

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