Rat Cardiac Fibroblasts Proliferation by Aldosterone

Objective To investigate the effect of aldosterone on proliferation of rat cardiac fibroblasts.Methods Upon the model of cultured rat FBs,aldosterone was used to stimulate proliferation of FBs,()~3H-Leucine(()~3H-Leu) and()~3H-Thymidine(()~3H-TdR) incorporation as the target to evaluate FBs proliferation.Results Synthesis rates of protein and nucleic acid of FBs stimulated by aldosterone increased in a dose dependent manner significantly in contrast to control(P0.01);spironoactone markedly inhibited syntheses of protein and nucleic acid mediated by aldosterone in FBs with a significant difference from aldosterone-stimulated group(P0.01).Conclusions Spironoactone inhibits the proliferation of FBs induced by aldosterone.