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2009•Xinxueguan kangfu yixue zazhiRequires access

Effect of aldosterone on the proliferation of cultured myocardiac fibroblasts

Jianhua Chen

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Abstract

Objective:To investigate the effect of aldosterone on the proliferation of cultured myocardiac fibroblasts in vitro.Methods:Myocardiac myofibroblasts derived from the heart of New Zealand rabbit were cultured.The 3rd passage of cells were divided into the following groups:aldosterone groups (in different concentration),aldosternoe+spironolactone group and control group.Both of cells metabolic activity and DNA synthesis were evaluated with WST-1 cleavage and BrdU incorporation assay (ELISA).Results:Compared with the control group,the OD values of both WST-1 and BrdU assay were significantly increased in a manner of dose-dependent pattern in aldosterone groups(P0.05).However,the OD of WST-1 and BrdU in aldosternoe+spironolactone group was lower than that in aldosterone groups(P0.01).Conclusion:Aldosterone can promotes the proliferation of cultured myocardiac fibroblasts in vitro.The effect of aldosterone can be blocked by spironolactone.

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Objective:To investigate the effect of aldosterone on the proliferation of cultured myocardiac fibroblasts in vitro.Methods:Myocardiac myofibroblasts derived from the heart of New Zealand rabbit were cultured.The 3rd passage of cells were divided into the following groups:aldosterone groups (in different concentration),aldosternoe+spironolactone group and control group.Both of cells metabolic activity and DNA synthesis were evaluated with WST-1 cleavage and BrdU incorporation assay (ELISA).Results:Compared with the control group,the OD values of both WST-1 and BrdU assay were significantly increased in a manner of dose-dependent pattern in aldosterone groups(P0.05).However,the OD of WST-1 and BrdU in aldosternoe+spironolactone group was lower than that in aldosterone groups(P0.01).Conclusion:Aldosterone can promotes the proliferation of cultured myocardiac fibroblasts in vitro.The effect of aldosterone can be blocked by spironolactone.

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Available abstract

Objective:To investigate the effect of aldosterone on the proliferation of cultured myocardiac fibroblasts in vitro.Methods:Myocardiac myofibroblasts derived from the heart of New Zealand rabbit were cultured.The 3rd passage of cells were divided into the following groups:aldosterone groups (in different concentration),aldosternoe+spironolactone group and control group.Both of cells metabolic activity and DNA synthesis were evaluated with WST-1 cleavage and BrdU incorporation assay (ELISA).Results:Compared with the control group,the OD values of both WST-1 and BrdU assay were significantly increased in a manner of dose-dependent pattern in aldosterone groups(P0.05).However,the OD of WST-1 and BrdU in aldosternoe+spironolactone group was lower than that in aldosterone groups(P0.01).Conclusion:Aldosterone can promotes the proliferation of cultured myocardiac fibroblasts in vitro.The effect of aldosterone can be blocked by spironolactone.

Key concepts: Aldosterone, Spironolactone, In vitro, Medicine, Internal medicine, Endocrinology, Myofibroblast, Biology

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