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Optimization of Fermentation Conditions for Exopolysaccharide Production by Bacillus amyloliquefaciens LPL061

Han Yuzhu

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Abstract

In order to improve expolysaccharide(EPS) production by Bacillus amyloliquefaciens LPL061,the medium and fermentation conditions were optimized by one-factor-at-a-time design and response surface analysis.The optimal culture medium consisted of 22 g/L sucrose and 18.4 g/L yeast extract at pH 7.0.The optimal fermentation conditions were 28 ℃,220 r/min shaking speed,3% inoculum size and 24 h.Under these conditions,the EPS yield was 4.46 g/L,1.51-fold higher than before the optimization.

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What this paper is about

In order to improve expolysaccharide(EPS) production by Bacillus amyloliquefaciens LPL061,the medium and fermentation conditions were optimized by one-factor-at-a-time design and response surface analysis.The optimal culture medium consisted of 22 g/L sucrose and 18.4 g/L yeast extract at pH 7.0.The optimal fermentation conditions were 28 ℃,220 r/min shaking speed,3% inoculum size and 24 h.Under these conditions,the EPS yield was 4.46 g/L,1.51-fold higher than before the optimization.

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Available abstract

In order to improve expolysaccharide(EPS) production by Bacillus amyloliquefaciens LPL061,the medium and fermentation conditions were optimized by one-factor-at-a-time design and response surface analysis.The optimal culture medium consisted of 22 g/L sucrose and 18.4 g/L yeast extract at pH 7.0.The optimal fermentation conditions were 28 ℃,220 r/min shaking speed,3% inoculum size and 24 h.Under these conditions,the EPS yield was 4.46 g/L,1.51-fold higher than before the optimization.

Key concepts: Bacillus amyloliquefaciens, Fermentation, Sucrose, Food science, Response surface methodology, Yeast extract, Yeast, Chemistry

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