Research on the Design of the Medium for the Amyloliquefaciens Bacillus Fermentation and Optimization of Its Fermentation Condition

[Objective] The scientific basis of the application of amyloliquefaciens bacillus was provided through the research.[Method] The design of the fermentation medium for a newly discovering amyloliquefaciens bacillus-SAB-1 was conducted for the optimal fermentation condition including the carbon and nitrogen source,the initial pH,the fermentation time and temperature,the inoculation amount and the amount of liquid in fermentation tank.[Results] From 8 different kinds of carbon and nitrogen sources,the long-term acting carbon source-soluble starch,the long-term acting nitrogen source-cottonseed meal,and the available carbon source-glucose,the available nitrogen source-yeast extracting solution were selected for its culturing.And the optimized fermentation condition in basal medium was as follows: the temperature,87.8℉;the speed,180 r/min;the fermentation time,26 hours;the initial pH value,6.0;the inoculation rate,1% and the liquid volume,50 ml/250 ml,under which condition,the fermentation yield of the bacillus strain was 46.0×109/ml.[Conclusion] The amyloliquefaciens bacillus fermentation in the design of condition reached basically to the demand of the scale industrial production.