Study on chemical constituents of Aletris spicata

Objective To study the chemical constituents of Aletris spicata. Methods The compounds were isolated and repeatedly purified by silica gel, TLC, and Sephadex LH-20 column chromatography as well as semi-Prep HPLC, and their structures were elucidated by means of spectral analysis and physicochemical properties. The antitumor and antibacterial activities of triterpenoids (compounds 1—3) were primarily screened. Results Nine compounds were obtained and identified as 24-methyl-9, 19-cyclolanost 24-en-3-ol (1), 24-methyl-9, 19-cyclolanost-25-en-3-ol (2), cycloneolitsol (3), americanol A (4), isoamericanol A (5), 9′-methyl americanol A (6), 1-(4′-hydroxyphenyl)-7-(3″-methoxyl-4″-hydroxyphenyl)-heptene-4-en-3-one (7), methyl-9, 12, 13-trihydroxy octadeca-10E, 15Z-dienoate (8), and 5-hydroxymethyl-2-furancarboxaldehyde (9). Conclusion All the compounds are isolated from the plants in Aletris L. for the first time. No anti-tumor and anti-bacterial activities are found in compounds 1—3.