ScholarLens Back to search
2012•Shandong Agricultural SciencesRequires access

Cloning and Sequence Analysis of cDNA Fragment of DFR Gene from Red-flower Pomegranate

Lijuan Feng

Open publisher page 1 citations

Abstract

Dihydroflavonol 4-reductase(DFR)is the key enzyme in the biosynthesis of anthocyanidins.With the total RNA from red-flower pomegranate Taishanhong as template and using degenerate primers,a new cDNA fragment of 446 bp was amplified by RT-PCR.The sequence analysis demonstrated that the deduced amino acid sequence shared 78%～81% homology with that of sweet cherry,grape and etc.These results suggested that the cloned cDNA fragment was from DFR gene of red-flower pomegranate,and its accession number in GenBank was JN316028.

About this research paper

What this paper is about

Dihydroflavonol 4-reductase(DFR)is the key enzyme in the biosynthesis of anthocyanidins.With the total RNA from red-flower pomegranate Taishanhong as template and using degenerate primers,a new cDNA fragment of 446 bp was amplified by RT-PCR.The sequence analysis demonstrated that the deduced amino acid sequence shared 78%～81% homology with that of sweet cherry,grape and etc.These results suggested that the cloned cDNA fragment was from DFR gene of red-flower pomegranate,and its accession number in GenBank was JN316028.

Why it matters

OpenAlex reports 1 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Dihydroflavonol 4-reductase(DFR)is the key enzyme in the biosynthesis of anthocyanidins.With the total RNA from red-flower pomegranate Taishanhong as template and using degenerate primers,a new cDNA fragment of 446 bp was amplified by RT-PCR.The sequence analysis demonstrated that the deduced amino acid sequence shared 78%～81% homology with that of sweet cherry,grape and etc.These results suggested that the cloned cDNA fragment was from DFR gene of red-flower pomegranate,and its accession number in GenBank was JN316028.

Key concepts: GenBank, Complementary DNA, Biology, Gene, Accession number (library science), Sequence analysis, Homology (biology), Genetics

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Cloning and Sequence Analysis of cDNA Fragment of DFR Gene from Red-flower Pomegranate — Research Paper | ScholarLens