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2013•Journal of Fujian Agriculture and Forestry UniversityRequires access

Cloning and sequence analysis of DFR gene from litchi

Zhao Zhi-chan

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Abstract

The dihydroflavonol 4-reductase(DFR) was a key enzyme in the anthocyanin biosynthesis.Degenerate primers designed according the conservative domain of dihydroflavonol 4-reductase(DFR) in plants were used to amplify the DFR fragments from the cDNA of litchi(Litchi chinensis Sonn.).A full length sequence of DFR gene was cloned from litchi pericarp by RACE(rapid-amplification of cDNA ends) method.The open reading frame of litchi DFR gene was 1062 bp,and encoding 353 amino acids.The 2321 bp length fragment was amplified from genome of litchi,which contained five introns respectively,in 119-255,426-1158,1353-1470,1632-1819,2013-2095 bp.It was found that the amino acid sequence of litchi DFR gene had closer relationship with mountain grape(Vitis amurensis),begonia(Malus hybrid cultivar) and mangosteen(Garcinia mangostana) through phylogenetic analysis.

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The dihydroflavonol 4-reductase(DFR) was a key enzyme in the anthocyanin biosynthesis.Degenerate primers designed according the conservative domain of dihydroflavonol 4-reductase(DFR) in plants were used to amplify the DFR fragments from the cDNA of litchi(Litchi chinensis Sonn.).A full length sequence of DFR gene was cloned from litchi pericarp by RACE(rapid-amplification of cDNA ends) method.The open reading frame of litchi DFR gene was 1062 bp,and encoding 353 amino acids.The 2321 bp length fragment was amplified from genome of litchi,which contained five introns respectively,in 119-255,426-1158,1353-1470,1632-1819,2013-2095 bp.It was found that the amino acid sequence of litchi DFR gene had closer relationship with mountain grape(Vitis amurensis),begonia(Malus hybrid cultivar) and mangosteen(Garcinia mangostana) through phylogenetic analysis.

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Available abstract

The dihydroflavonol 4-reductase(DFR) was a key enzyme in the anthocyanin biosynthesis.Degenerate primers designed according the conservative domain of dihydroflavonol 4-reductase(DFR) in plants were used to amplify the DFR fragments from the cDNA of litchi(Litchi chinensis Sonn.).A full length sequence of DFR gene was cloned from litchi pericarp by RACE(rapid-amplification of cDNA ends) method.The open reading frame of litchi DFR gene was 1062 bp,and encoding 353 amino acids.The 2321 bp length fragment was amplified from genome of litchi,which contained five introns respectively,in 119-255,426-1158,1353-1470,1632-1819,2013-2095 bp.It was found that the amino acid sequence of litchi DFR gene had closer relationship with mountain grape(Vitis amurensis),begonia(Malus hybrid cultivar) and mangosteen(Garcinia mangostana) through phylogenetic analysis.

Key concepts: Garcinia mangostana, Gene, Complementary DNA, Biology, Open reading frame, Cloning (programming), Sequence analysis, Intron

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