The content of the total flavonoids of Brassicarapa L by spectrophotometric method

Objective To establish the optimum procedure of the flavonoidS extraction of Brassica rapa L.To Brassica rapa L leaves the rational development and utilization to provide a scientific basis.Methods Ethanol as solvent,the use of ultrasonic extraction,colorimetric method determination of total flavonoids in mulberry leaves drug content,and stability,precision and recovery test of the method of testing the reliability and reproducibility.Results Standard solution and test solution for the department in the maximum absorption 510nm.Regression equation: A=0.01853 c+0.07628,r=0.9998.The average content of total flavonoids: 2.797%,with an average recovery rate: 101.6%.Precision and recovery values of RSD are as follows: 0.85%、1.21%.This method is simple,sensitive and can be used just Brassica rapa L determination of total flavonoids.