Establishment of determination method of total flavonoids in Hippophe rhamnoids L leaves

A method for determination of total flavonoids in Hippophe rhamnoids L leaves is established,by singlefactor screening, multiple-factor orthogonal test and methodology examination of NaNO2-Al(NO3)3-NaOH system spectrophotometry.The single factor includs reaction volume and reaction time.The results show that the optimization methods are:Take 1mL sample solution in the beaker,add70% ethanol 1mL and 5% NaNO20.5mL,shake well,react 6min.Then add 10% Al(NO3)30.5mL,shake well,react 6 min;After that add 4% NaOH 6 mL,shake well,react 15 min,determine absorbance at 510 nm with 70% ethanol as reference solution.The methodology shows that the method has good stability and reproducibility,high precision and recovery.It is more suitable for the determination content of total flavonoids in Hippophe rhamnoids L leaves,which has simple equipment,convenient operation and high accurate.using this method,the content of total flavonoids is 2.049%,which is 1.337 times than before optimized.Both of then reach to the regulation standard of China pharmacopoeia.This method can be looked as a reference method for the determination of total flavonoids in other materials.