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A Study on Tissue Culture of Tender Bud of Buddleja cylindrostachya

Wenling Guan

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Abstract

Taking the tender bud as the explant,four stages of tissue culture of B.cylindrostachya namely induction,proliferation,subculture and rooting culture were studied.The experimental results showed that the best method for disinfection was soaking the explant in mercuric chloride of 0.1 % for 7 minutes.The best media for explant induction,bud proliferation,subculture and rooting induction were MS+6-BA 0.5 mg/L+NAA 0.02 mg/L,MS+6-BA 0.5 mg/L+NAA 0.02 mg/L,MS+6-BA 0.5 mg/L and MS+6-BA 0.5 mg/L+NAA 0.02 mg/L+AC 0.3 % respectively.

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What this paper is about

Taking the tender bud as the explant,four stages of tissue culture of B.cylindrostachya namely induction,proliferation,subculture and rooting culture were studied.The experimental results showed that the best method for disinfection was soaking the explant in mercuric chloride of 0.1 % for 7 minutes.The best media for explant induction,bud proliferation,subculture and rooting induction were MS+6-BA 0.5 mg/L+NAA 0.02 mg/L,MS+6-BA 0.5 mg/L+NAA 0.02 mg/L,MS+6-BA 0.5 mg/L and MS+6-BA 0.5 mg/L+NAA 0.02 mg/L+AC 0.3 % respectively.

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Available abstract

Taking the tender bud as the explant,four stages of tissue culture of B.cylindrostachya namely induction,proliferation,subculture and rooting culture were studied.The experimental results showed that the best method for disinfection was soaking the explant in mercuric chloride of 0.1 % for 7 minutes.The best media for explant induction,bud proliferation,subculture and rooting induction were MS+6-BA 0.5 mg/L+NAA 0.02 mg/L,MS+6-BA 0.5 mg/L+NAA 0.02 mg/L,MS+6-BA 0.5 mg/L and MS+6-BA 0.5 mg/L+NAA 0.02 mg/L+AC 0.3 % respectively.

Key concepts: Explant culture, Subculture (biology), Tissue culture, Biology, Botany, Horticulture, In vitro, Biochemistry

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