Study on the Tissue Culture and Rapid Multiplication of Kerria japonica

[Objective] The research aimed to improve the nursery propagation rate of Kerria japonica.[Method] Tender stem of K.japonica was used as explants to investigate the rapid propagation technology.[Result] In MS+2.0 mg/L 6-BA+0.2 mg/L NAA medium,the number of clustered bud differentiation was the highest,the differentiation rate reached to 100%,and the quality of clustered bud was the best;In MS+1.0 mg/L 6-BA+0.5 mg/L KT+ 0.1 mg/L NAA medium,the proliferation multiples of the bud was the highest,reached 11.87;1/2 MS+0.1 mg/L NAA medium was the best for rooting,there were 3-5 roots in every tender stem;the transplanted survival rate was 96%.[Conclusion] MS+2.0 mg/L 6-BA+0.2 mg/L NAA was the optimal medium to induce clustered bud during tender stem culture;The best medium for subculture was MS+1.0 mg/L 6-BA+0.5 mg/L KT+0.1 mg/L NAA,and medium 1/2 MS+0.1 mg/L NAA have a better effect on regeneration seedlings rooting.