Enzyme-assisted Supercritical CO_2 Extraction of Lycopene
Duan Xu
Abstract
Duan Xu
Abstract
Single factor method and orthogonal array design were adopted to explore the optimal enzyme-assisted supercritical CO2 extraction process of lycopene from tomato.Results revealed that the optimal processing conditions for degrading cell wall with pectinase and cellulose were 4% of pectinase amount and 3% of cellulase amount, 1.5 h of reaction at 40 ℃ and pH 4.0, and the optimal processing conditions for supercritical CO2 extraction were 15 MPa of extraction pressure and 4 h of extraction at 40 ℃.
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Single factor method and orthogonal array design were adopted to explore the optimal enzyme-assisted supercritical CO2 extraction process of lycopene from tomato.Results revealed that the optimal processing conditions for degrading cell wall with pectinase and cellulose were 4% of pectinase amount and 3% of cellulase amount, 1.5 h of reaction at 40 ℃ and pH 4.0, and the optimal processing conditions for supercritical CO2 extraction were 15 MPa of extraction pressure and 4 h of extraction at 40 ℃.
Key concepts: Pectinase, Cellulase, Extraction (chemistry), Supercritical fluid, Lycopene, Chromatography, Chemistry, Supercritical carbon dioxide