Protective effect of bilobalide on vascular dementia mice

Objective To observe the effect of bilobalide(BB)on the function of learning and memory of vascular dementia mice.Methods Bilateral commoncarotid arteries ligation-reperfusion combined with tail end depletion method was used to establish VD model.The mice were randomly divided into 9 subgroups:sham surgery group,model group,Nimotop group,Huperzine A group,BB(1.25,12.5,5,10,50 mg·kg-1)group,continuous administration 60 days after surgery.Learning and memory ability of mice in treatment group was observed by step-down test and step-through test;acetylcholinesterase(AChE)and SOD activity,MDA and NO in the brain were determined by chemical colorimetry.The changes of pathomorphology were observed by HE staining.Results BB could shorten the reaction time of learning,prolong the latent time of memory,reduce times of mistakes,raise the SOD activity of brain tissue,decrease MDA and NO content,restrain the activity of AchE.The pathology results showed that there was an improvement in the pathological changes of cortex and hippocampus CA1 of vascular dementia mice brain tissue.Conclusion BB has a protective effect on vascular dementia mice.