2013•Journal of Jining Medical UniversityRequires access

Comparative study on bacteriostatic efficacy of honeysuckle and wild chrysanthemum

Xiaoping Cheng

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Abstract

Objective The bacteriostatic efficacy of alcohol extract of honeysuckle and wild chrysanthemum were investigated in this work.Methods Their reaction was observed and compared by experiments with Paper Dish methods.Results The diameters of bacterial inhibition zones of two bacteria such as Staphylococcus aureus and Subtilis Bacillus to the ethanol extract containing honeysuckle crude drug 492mg/ml were larger than 8mm,but the diameters of bacterial inhibition zones of Escherichia coliand Bacillus to the ethanol extract containing honeysuckle crude drug 492mg/ml were smaller than 8mm.The diameters of bacterial inhibition zones of two bacteria such as Staphylococcus aureus and Escherichia coliand Bacillus to the ethanol extract containing wild chrysanthemum drug 1035mg/ml were larger than 8mm,but the diameters of bacterial inhibition zones of Subtilis Bacillus to the ethanol extract containing wild chrysanthemum drug 1035mg/ml were smaller than 8mm.The minimal inhibitory concentration was 1∶8,such as honeysuckle alcohol extract to Staphylococcus aureus and Subtilis Bacillus.The minimal inhibitory concentration was 1∶4 such as honeysuckle alcohol extract to the E.coli.The minimal inhibitory concentration was 1∶64 such as wild chrysanthemum alcohol extract to the E.coli.The minimal inhibitory concentration was 1∶16 such as wild chrysanthemum alcohol extract to the Staphylococcus aureus.The minimal inhibitory concentration was 1∶4 such as wild chrysanthemum alcohol extract to the Subtilis Bacillus.Conclusion The honeysuckle extract has obvious bacteriostatic effect to Staphylococcus aureus and Subtilis Bacillus,but the honeysuckle extract has no inhibitory effect to Escherichia coli and Bacillus.The wild chrysanthemum extract has obvious bacteriostatic effect to Staphylococcus aureus,Escherichia coli and Bacillus,but the honeysuckle extract has no inhibitory effect to Subtilis Bacillus.

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Objective The bacteriostatic efficacy of alcohol extract of honeysuckle and wild chrysanthemum were investigated in this work.Methods Their reaction was observed and compared by experiments with Paper Dish methods.Results The diameters of bacterial inhibition zones of two bacteria such as Staphylococcus aureus and Subtilis Bacillus to the ethanol extract containing honeysuckle crude drug 492mg/ml were larger than 8mm,but the diameters of bacterial inhibition zones of Escherichia coliand Bacillus to the ethanol extract containing honeysuckle crude drug 492mg/ml were smaller than 8mm.The diameters of bacterial inhibition zones of two bacteria such as Staphylococcus aureus and Escherichia coliand Bacillus to the ethanol extract containing wild chrysanthemum drug 1035mg/ml were larger than 8mm,but the diameters of bacterial inhibition zones of Subtilis Bacillus to the ethanol extract containing wild chrysanthemum drug 1035mg/ml were smaller than 8mm.The minimal inhibitory concentration was 1∶8,such as honeysuckle alcohol extract to Staphylococcus aureus and Subtilis Bacillus.The minimal inhibitory concentration was 1∶4 such as honeysuckle alcohol extract to the E.coli.The minimal inhibitory concentration was 1∶64 such as wild chrysanthemum alcohol extract to the E.coli.The minimal inhibitory concentration was 1∶16 such as wild chrysanthemum alcohol extract to the Staphylococcus aureus.The minimal inhibitory concentration was 1∶4 such as wild chrysanthemum alcohol extract to the Subtilis Bacillus.Conclusion The honeysuckle extract has obvious bacteriostatic effect to Staphylococcus aureus and Subtilis Bacillus,but the honeysuckle extract has no inhibitory effect to Escherichia coli and Bacillus.The wild chrysanthemum extract has obvious bacteriostatic effect to Staphylococcus aureus,Escherichia coli and Bacillus,but the honeysuckle extract has no inhibitory effect to Subtilis Bacillus.

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Available abstract

Objective The bacteriostatic efficacy of alcohol extract of honeysuckle and wild chrysanthemum were investigated in this work.Methods Their reaction was observed and compared by experiments with Paper Dish methods.Results The diameters of bacterial inhibition zones of two bacteria such as Staphylococcus aureus and Subtilis Bacillus to the ethanol extract containing honeysuckle crude drug 492mg/ml were larger than 8mm,but the diameters of bacterial inhibition zones of Escherichia coliand Bacillus to the ethanol extract containing honeysuckle crude drug 492mg/ml were smaller than 8mm.The diameters of bacterial inhibition zones of two bacteria such as Staphylococcus aureus and Escherichia coliand Bacillus to the ethanol extract containing wild chrysanthemum drug 1035mg/ml were larger than 8mm,but the diameters of bacterial inhibition zones of Subtilis Bacillus to the ethanol extract containing wild chrysanthemum drug 1035mg/ml were smaller than 8mm.The minimal inhibitory concentration was 1∶8,such as honeysuckle alcohol extract to Staphylococcus aureus and Subtilis Bacillus.The minimal inhibitory concentration was 1∶4 such as honeysuckle alcohol extract to the E.coli.The minimal inhibitory concentration was 1∶64 such as wild chrysanthemum alcohol extract to the E.coli.The minimal inhibitory concentration was 1∶16 such as wild chrysanthemum alcohol extract to the Staphylococcus aureus.The minimal inhibitory concentration was 1∶4 such as wild chrysanthemum alcohol extract to the Subtilis Bacillus.Conclusion The honeysuckle extract has obvious bacteriostatic effect to Staphylococcus aureus and Subtilis Bacillus,but the honeysuckle extract has no inhibitory effect to Escherichia coli and Bacillus.The wild chrysanthemum extract has obvious bacteriostatic effect to Staphylococcus aureus,Escherichia coli and Bacillus,but the honeysuckle extract has no inhibitory effect to Subtilis Bacillus.

Key concepts: Honeysuckle, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Microbiology, Ethanol, Minimum inhibitory concentration, Biology

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