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2008•Journal of East China Institute of TechnologyRequires access

Determination of Ethyl 2-oxocyclopentanecarboxylate by Reversed Phase High Performance Liquid Chromatograph

Lijuan Xu

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Abstract

A method for the determination of ethyl 2-oxocylopentanecaroxylate by reversed phase high performance liquid chromatography is developed. Kromasil C18 (250 mm×4.6 mm, 5 μm) is chosen as chromatographic column. Methanol-water (60∶40, V∶V) at flow-rate of 0.6 mL/min is mobile phase with UV detection wave at 253 nm. The relationship between peak height and concentration in the range from 6 to 300 μg/mL show good linearity. RSD is 3.30%.The detection limit is 0.08μg/mL.The recovery of samples are 98.02%~101.22%. It indicates that the proposed method is sensitive reliable and can be?applied in analysis of medical materials.

About this research paper

What this paper is about

A method for the determination of ethyl 2-oxocylopentanecaroxylate by reversed phase high performance liquid chromatography is developed. Kromasil C18 (250 mm×4.6 mm, 5 μm) is chosen as chromatographic column. Methanol-water (60∶40, V∶V) at flow-rate of 0.6 mL/min is mobile phase with UV detection wave at 253 nm. The relationship between peak height and concentration in the range from 6 to 300 μg/mL show good linearity. RSD is 3.30%.The detection limit is 0.08μg/mL.The recovery of samples are 98.02%~101.22%. It indicates that the proposed method is sensitive reliable and can be?applied in analysis of medical materials.

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Available abstract

A method for the determination of ethyl 2-oxocylopentanecaroxylate by reversed phase high performance liquid chromatography is developed. Kromasil C18 (250 mm×4.6 mm, 5 μm) is chosen as chromatographic column. Methanol-water (60∶40, V∶V) at flow-rate of 0.6 mL/min is mobile phase with UV detection wave at 253 nm. The relationship between peak height and concentration in the range from 6 to 300 μg/mL show good linearity. RSD is 3.30%.The detection limit is 0.08μg/mL.The recovery of samples are 98.02%~101.22%. It indicates that the proposed method is sensitive reliable and can be?applied in analysis of medical materials.

Key concepts: Chromatography, Detection limit, Volumetric flow rate, High-performance liquid chromatography, Methanol, Phase (matter), Chemistry, Linearity

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Determination of Ethyl 2-oxocyclopentanecarboxylate by Reversed Phase High Performance Liquid Chromatograph — Research Paper | ScholarLens