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2006•Journal of Biological ControlRequires access

Determination of Wanlongmycin by High Performance Liquid Chromatography

Huifang Shen

Open publisher page 2 citations

Abstract

The method for the separation and determination of Wanlongmycin in fermentation broth was developed.A C18 reversed column(3.9mm×150mm) was used.The column temperature was 40 ℃.The mobile phase was methanol-water(20∶80).The flow rate was 1.0ml/min and the detection wavelength was UV 280nm.The results showed that the active constituent Wanlongmycin can be separated effectively.In linear range 17.5-280μg/ml,there was a regressive equation y=17343x-164139,the correlation coefficient was 0.9998(n=5).

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What this paper is about

The method for the separation and determination of Wanlongmycin in fermentation broth was developed.A C18 reversed column(3.9mm×150mm) was used.The column temperature was 40 ℃.The mobile phase was methanol-water(20∶80).The flow rate was 1.0ml/min and the detection wavelength was UV 280nm.The results showed that the active constituent Wanlongmycin can be separated effectively.In linear range 17.5-280μg/ml,there was a regressive equation y=17343x-164139,the correlation coefficient was 0.9998(n=5).

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Available abstract

The method for the separation and determination of Wanlongmycin in fermentation broth was developed.A C18 reversed column(3.9mm×150mm) was used.The column temperature was 40 ℃.The mobile phase was methanol-water(20∶80).The flow rate was 1.0ml/min and the detection wavelength was UV 280nm.The results showed that the active constituent Wanlongmycin can be separated effectively.In linear range 17.5-280μg/ml,there was a regressive equation y=17343x-164139,the correlation coefficient was 0.9998(n=5).

Key concepts: Chromatography, Correlation coefficient, Linear correlation, Methanol, Volumetric flow rate, High-performance liquid chromatography, Linear range, Linear relationship

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